Cell gene expression analysis is promising to characterize cell heterogeneity, but the current approach is compromised in terms of coverage, sensitivity, or throughput.
Here, let's introduce Smart-
Seq2 with improved reverse transcription, template switching and pre-amplification to increase the yield and length of libraries from individual cells. Smart-
Seq2 transcript library improved detection, coverage, bias, and accuracy compared to Smart-
Seq library and use off-generatethe-
Lower cost shelf reagents.