high resolution ftir imaging provides automated discrimination and detection of single malaria parasite infected erythrocytes on glass - high resolution document camera

New highly sensitive malaria diagnostic tools are urgently needed to detect infections in asymptomatic carriers and patients with hypoparasite.
In order to find a highly sensitive diagnostic tool that can identify parasitic infection at the single cell level, we have been exploring Fourier transform infrared (FTIR)
A microscope using a focal plane array (FPA)
Imaging Detector
Here, for the first time, we report on the application of a new optical configuration developed by Agilent, which contains an objective cassegren optical system with a 25 x condenser and a high numerical aperture (NA = 0. 81)
And an extra high-fold optical system that provides 0 in the microscope.
66 micron pixel resolution (
Total magnification of the infrared system of 61 ×)
Diagnosis of malaria parasites at single cell level on traditional glass microscope slides.
High quality images clearly address the digestive bubble of the parasite
Cellular resolution using this method.
In addition, we developed an algorithm that first detects cells in an infrared image and then extracts the mean spectrum.
Then, the average spectrum runs through a model based on partial least squares-
Discrimination analysis (PLS-DA)
Clear diagnosis of infection from normal cells.
High-quality images, and the fact that this measurement can be achieved without a synchronous accelerator source on traditional slides, show the prospect of being a potential gold standard for malaria detection at single cell levels.