loading

A Professional Manufacturer of Smart Interactive Screens For More Than 10 Years 

the interface of oxytocin-labeled cells and serotonin transporter-containing fibers in the primate hypothalamus: a substrate for ssris therapeutic effects? - drawing tablet reviews

the interface of oxytocin-labeled cells and serotonin transporter-containing fibers in the primate hypothalamus: a substrate for ssris therapeutic effects?  -  drawing tablet reviews

Oxytocin (OT)
By the room (PVN)
Regarded as nuclear (SON)
Lower Hill.
Although OT is known for its role in milk
Recent studies have shown that OT is involved in the expression of social behavior, memory processing, regulation of fear and stress response.
Proof that OT affects accessory behavior (such as parental care and reproduction) and reduces anxiety leads to speculation that it may play a role in mood disorders.
Evidence from pharmacology studies indicates that the OT system is regulated by chloramine and believes that OT is an intermediary of the chloramine re-absorption inhibitor (SSRIs)
Anti-depression drugs
In this study, we studied OT-
Labeled cells and five serotonin transporters (5-HTT)
Immune Response (IR)
Using biochemistry and double-
Immune fluorescence technology
Consistent with previous reports, distribution of OT
Labeled cells in the lower part of the visual Hill are limited to PVN and SON.
In these nuclei, we prove that 5-HTT-
Label fibers follow the distribution of OT-labeled cells. Overlap of OT-
Labeled neurons and 5-HTT-
IR fibers appear next to cells, between cells, in the back, and in the subarea of the rear of PVN. In the SON, 5-HTT-
Label fiber and OT
The labeled cells overlap in the medial segment of the abdomen and the "SAC" section of the dorsolum SON.
These findings provide neuroanatomical support for ssris's therapeutic effects on social and anxiety, which may be partially mediated by the components of the OT system. One female (MFJ7)
Five men (
MNJ1, MNJ2, MNJ3, MNJ5, MNJ6)
Used in these studies.
All the experiments were conducted in accordance with the guidelines of the National Institutes of Health.
The experimental design and techniques were designed to minimize the use and pain of animals and were reviewed and approved by the University of Rochester Animal Research Committee.
The initial anesthesia was to inject ketamine ketone through the muscles (10u2009mg/kg)
, And intravenous injection of e-obarbital (
Initial dose 20 mg/kgv.
And maintain as needed).
The animals were subjected to deep anesthesia and were put to death after heart perfusion with normal saline containing 0.
Heparin sulfate of 5 ml, followed by 0 in more than 4% formaldehyde solutions.
1 µm phosphate buffer (pH 7. 4).
Then take out the brain and freeze protection with an increase in sucrose gradient (10, 20 and 30%).
Cut a continuous slice of 50 μm on the frozen slicer and transfer to 0.
1 m phosphate buffer or solution of low temperature protective agent.
Sections of adjacent compartments were selected for immune staining with OT (
Hudson star, Hudson, WI, 1: 10 pmand 5-HTT (
Monoclonal antibody technology
, Stone Mountain, GA, mouse monoclonal 1: 150 2016000)
Staining of antibodies and phenol Violet.
Also selected a compartment for 5-
Comparison of HT immune staining with 5-HTT IR (
Immune star, Hudson, WI, 1:100, 000, rabbit, rabbit).
Dilution curves were performed before the actual experiment to determine the optimal concentration of each primary antibody.
The control sections that omitted primary antibodies did not result in IR staining of cells or fibers.
Rinse the slices thoroughly first in 0.
1 µm phosphate buffer (pH 7. 2)with 0. 3% Triton X-100 (PB-T).
After treatment with endogenous peroxide inhibitors (EPI), and more PB-
T rinse, tissue pre-incubated in 10% normal goat serum (NGS)
Dilute with PBT (NGS-PB-T)for 30u2009min. In the 5-HTT and 5-
In HT experiments, 1% of bovine serum was added to the NGS to reduce background staining.
Then put the tissue into the primary resistancesera to OT, 5-HTT, and 5-
HT was incubated for 4 nights at 4 °c.
After washing with PB-
T, incubated the slices with the appropriate biomarker secondary antibody and used avinitin-
Biotin complex reaction (
Vector lab, Vector lab, Vectastatin ABC kit, Burlingame, CA).
The staining was enhanced by incubation of 1-3 min in 3,3′ diamino B 4 salt (DAB)and 0.
03% hydrogen peroxide.
The slices are mounted on sub-bed slides, dehydrated in an increasing alcohol gradient, cleared in PX, and the lid slides.
Several immune-stained compartments were re-stained with phenol purple.
Single analysis-
The adjacent part of the mark, an extra compartment for a woman (MFJ7)and two male (MFJ2 and JFJ5)
The monkey was selected as a double label for OT and 5-HTT. For double-
Using the DAB mark, the slices of OT and nickel are processed first
Strengthen in OT to produce blue-black reaction productlabeled cells.
5-2 processing parts
HTT without nickel reinforcement gives a light brown label of 5-HTT-Positive fibers.
Dilution curves were first performed in a single-label fluorescence assay to optimize the fluorescence signal and minimize background staining.
After treatment with EPI, incubated in 10% NGS and incubated in mixed OT for four nights (
Rabbit, 1:10and 5-HTT (Mouse, 1: 75 000)
Antibody at 4 °c
Then, thoroughly clean the tissue in PB-
TX, incubated in 10% NGSPB-
TX 30 min, incubated dark for four hours in filtered Alexafluor 546 (anti-
Rabbit immunoglobulin antibody
And Alexafluor 488 (anti-
Mouse IgG antibody(
Molecular probe company, Eugene, OR)
, With OT and 5-
HTT resistance, respectively.
Control experiment of fluorescent secondary antibody being "reversed (
Such as Alexafluor 546 defense
5-mouse IgG antibody
HTT 1 resistance;
Alexafluor 488 defense
Rabbit IgG antibody against OT 1)
This is also done to ensure a similar label mode.
The tissue is then rinsed for 1 Thanh, mounted on the slide and covered in the water medium (
Vector lab). For DAB-
Immune response compartment, distribution of OT
The labeled cells in the lower Hill are hands-
Draw in bright
Use a stretch tube attached to the microscope for on-site lighting (× 10 objective). Hand-
Then enter the drawn chart into the computer using the application Canvas 5. 0 (
Victoria ACD Systems, BC, Canada)
Used with a drawing tablet (
Wacom TechnologyVancouver, Washington). 5-HTT-
The fiber of the label is handmade.
Drawing under bright and dark lighting, also using camera lucida technology (× 10 objective).
Then, use the program Adobe Photoshop 6 to scan a paper chart of the marked fibers and surrounding landmarks, including fiber bundles and blood vessels, into the computer. 0 (300u2009dpi)(Adobe Inc. , San Jose, CA)
And import into the drawing program Canvas 5. 0.
Finally, the boundary between PVN and SON in the adjacent Nissl-
The dyed part is also drawn, input the computer with the help of the drawing tablet and in OT and 5-HTT-
Part of the mark.
Landmarks such as blood vessels and fiber bundles are used for alignment. 5-HTT and OT-
In order to confirm these relationships, the labeled slices that were re-dyed with the phenol Violet were examined.
Slices with 5-immune response
Evaluation of 5-with HTT and phenol Violet re-dyeing-
HTT staining of trans-subnuclear.
Double the additional part
Marked as the immune response of OT and five serotonin transporter (5-HTT-IR)
The use of DAB technology was studied at high power using oil immersion.
The analyzed DAB-
Label section, additional section double-labeled for 5-
HTT/OT with immune fluorescent antibodies was evaluated using a fluorescence microscope to further confirm the relationship between these markers.
Use the Optronic Microfire color CCD to take the image and format it in Adobe Photoshop.
In addition, Z-is used using a co-focused laser microscope-Stack analysis (
2 μm distance between pieces)(
Leica Microsystems, Exton, PA).

GET IN TOUCH WITH Us
recommended articles
Knowledge INFO CENTER FAQ
10 Hidden Features of Interactive Flat Panels You Didn’t Know Could Save Time & Money
Interactive Flat Panels (IFPs) have revolutionized classrooms, boardrooms, and collaborative spaces worldwide. While most users focus on their basic functions—touchscreen displays and presentation tools—these powerful devices are packed with underutilized features that can dramatically streamline workflows and cut costs. Below, we unveil 10 hidden gems that turn your IFP into a productivity powerhouse.
Interactive Flat Panel vs. Traditional Whiteboards: Which Saves More Time and Money?
In today’s evolving educational and corporate landscapes, choosing the right collaboration tool is critical. While traditional whiteboards have been classroom and boardroom staples for decades, interactive flat panels (IFPDs) are increasingly seen as the future of dynamic collaboration. But when it comes to saving time and money, which option truly delivers? Let’s break down the financial and operational impacts of both tools to help you decide.
How to Choose the Perfect Interactive Flat Panel: 7 Essential Factors for 2025
In today’s tech-driven world, interactive flat panels (IFPDs) have become indispensable tools for modern classrooms, boardrooms, and collaborative spaces. Whether you’re upgrading your office or building a smart classroom, selecting the right IFPD can transform productivity and engagement. Here’s a streamlined guide to help you make an informed decision:
The Interactive Flat Panel Revolution: How Smart Collaboration is Redefining Work & Education
Imagine a world where classrooms buzz with holographic science experiments, boardrooms transform into immersive war rooms with global teams, and hospital staff troubleshoot emergencies on a digital canvas – all through a single device. This isn’t sci-fi; it’s the reality powered by next-gen Interactive Flat Panels (IFPs). Forget “digital whiteboards” – today’s IFPs are AI-driven collaboration hubs rewriting human interaction. Let’s explore why they’re becoming the spine of modern workspaces and learning ecosystems.
Why Smart Board Prices Are Worth It: Smarter Tech for Modern Learning  
When considering white board smart board prices, it’s not just about cost—it’s about value. Modern LCD display infrared smart whiteboards are packed with features that transform classroom teaching, e-learning, and remote collaboration. Here’s why ITATOUCH smart boards stand out……
Maintenance Tips for Long-Lasting ITATOUCH Interactive Flat Panels
Interactive flat panels (IFPs) like the ITATOUCH Interactive Flat Panel have revolutionized collaboration and learning in both educational and corporate environments. These high-tech tools empower educators to deliver dynamic lessons and enable businesses to streamline presentations and brainstorming sessions. However, to ensure peak performance and longevity, proper maintenance is essential. Below are practical tips to keep your ITATOUCH panel running smoothly for years to come.
Why Schools Are Upgrading to Interactive Flat Panels: A Guide for Educators
ITATOUCH interactive flat panels are highly rated, with over 99% of clients across 50+ countries giving "Good" to "Very Good" reviews. They integrate seamlessly with platforms like Google Classroom, Zoom, and Microsoft Teams, offering reliable, innovative tools for educators. ITATOUCH provides custom solutions, including OEM, ODM, SKD, and fully customized options, all competitively priced and certified (TUV-CB, CE, FCC, ROHS, ISO). With fast delivery (3-15 days) and readily available stock, ITATOUCH ensures minimal downtime. Upgrade your classroom with ITATOUCH and embrace the future of education.
After the splicing screen is added with the touch frame, the large screen interaction can be realized, which can be better introduced and publicized by adding the touch frame, and the splicing screen can have the effect of realizing multi-person interactive touch wall, therefore, after the touch frame is added, the use of the splicing screen is more abundant, the configuration requirements for the splicing screen system are lower, and the flexible space for installing the touch frame is larger,
The teaching all-in-one machine must be familiar to everyone, from the earlier computer projector to the present interaction and teaching.
In this era of continuous influx of information technology, the intelligent touch all-in-one machine is well known as the fifth media besides paper media, radio, television and the Internet.
no data
Established in Oct, 2016, focusing on the interactive high-tech products, specializing in the development of LCD touch screen monitor, interactive whiteboard, interactive learning software, infrared touch screen frame, interactive tablet … etc.
INFORMATION FOR INQUAIRY
Tel: +86 755 28281849
Wechat & whatsapp: +86 13582949978
Address:  Building #123, Mansheng Industrial District, Gongming Town, Guangming District, Shenzhen, China
Copyright © 2022 ITATOUCH| Sitemap
Customer service
detect